目的 为铁皮石斛种质的评价和选育提供科学依据。方法 测量农艺性状指标;分析茎化学成分;观察植物与菌株M2(Mycena sp.)菌根栽培过程中生物量和化学成分含量的动态变化。结果 两种表型铁皮石斛的茎长、节距、叶宽和叶形指数有显著差异(P<0.05);醇溶性浸出物和多糖含量有显著差异(P<0.05);在A型与B型的生长过程中4,4′-二羟基-3,5-二甲氧基联苄和3,4′-二羟基-5-甲氧基联苄的含量有很大差异。菌根栽培12月,M-A组的茎干重和全株干重均极显著高于CK-A组(P<0.01),分别提高了37.6%和37.3%,茎多糖含量提高了36.8%,6个对照品化合物含量提高了5%~50%。菌根栽培12月,M-B组茎多糖含量提高了16.7%,6个对照品化合物含量提高了25%~170%。结论 两种表型铁皮石斛的联苄含量,以及菌根栽培后它们对同一菌根真菌的响应能力有差异。
Abstract
OBJECTIVE To provide scientific basis for variety evaluation and selection of Dendrobium officinale. METHODS The agronomic traits of D. officinale were measured. The chemical compositions of D. officinale stem were analyzed. The dynamic changes of plant biomass and chemical composition content during mycorrhizal cultivation with M2 (Mycena sp.)were observed. RESULTS There were significant differences between D. officinale phenotype A and B in stem length, internode length, leaf width and leaf shape index (P<0.05), and in the content of polysaccharide and ethanol soluble extract (P<0.05). Moreover, the contents of 4,4′-dihydroxy-3,5-dimethoxybibenzyl and 3,4′-dihydroxy-5-methoxybibenzyl were always very different between phenotype A and B during plant growth. After 12 months of growth, the stem dry weight and plant dry weight of M-A group were significantly higher than those of CK-A group(P<0.01), increasing by 37.6% and 37.3%, respectively. The contents of polysaccharide and six marker compounds of M-A group were increased by 36.8% and 5%-50% than those in CK-A group, respectively. After 12 months of growth, the contents of polysaccharide and six marker compounds of M-B group were increased by 16.7% and 25%-170% than those in CK-B group, respectively. CONCLUSION The bibenzyl content and the response ability to mycorrhizal fungi M2 of D. officinale phenotype A and B are different.
关键词
铁皮石斛 /
农艺性状 /
联苄 /
多糖 /
菌根真菌
{{custom_keyword}} /
Key words
Dendrobium officinale /
phenotype /
bibenzyl /
polysaccharide /
mycorrhizal fungi
{{custom_keyword}} /
中图分类号：
R282
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P(2015)Vol Ⅰ (中国药典2015年版. 一部)[S]. 2015:282-283.
[2] HE D G,YANG L T,LI Y R,et al. Antioxidant activity of crude and purified polysaccharide from suspension cultured protoconmns of Dendrobium candidum in vitro[J]. Chin Tradit Pat Med (中成药),2007,29(9):1265-1269.
[3] ZHANG H Y,DAI Y G,MA C,et al. Effects of polysaccharide from Dendrobium officinale Kiruma et Migo on the immune function of mice S180 sarcoma[J]. Zhejiang J Tradit Chin Med(浙江中医杂志),2009,44(5):381-382.
[4] FAN C Y. Research on Dendrobium officinale Kiruma et Migo different cultivation quality evaluation[D]. Wenzhou: Wenzhou University, 2014.
[5] YANG L,WANG H J,SHI S,et al. Fingerprints and heavy metal contents of Dendrobium officinale produced from 12 areas [J]. Acta Bot Boreali-Occiden Sin (西北植物学报),2014,34(10):2078-2084.
[6] ZHANG G B,ZHANG J G,HAN X X,et al. Wild Dendrobium officinale main phenotypic traits and polysaccharide content[J]. J Zhejiang Agricul Sci(浙江农业科学),2015,56(9):1406-1408.
[7] ZHU Y,SI J P,GUO B L,et al. Regularity of polysaccharide content variation artificial cultivation of Dendrobium officinale[J]. China J Chin Mater Med (中国中药杂志),2010,35(4):427-430.
[8] ZHANG B B,GONG X Y,HUANG Z,et al. Determination of active ingredient contents in different parts of red and green stems of Dendrobimn officinale Kimura et Migo [J]. Chin Arch Tradit Chin Med (中华中医药学刊),2016,34(7):1743-1745.
[9] JIANG C Y. Variations of total flavonoids and polysaccharide concentrations in leaf of Cyclocarya paliurus (Betal.) Iljinskaya with different morphological traits[D]. Nanchang: Jiangxi Agricultural University, 2015.
[10] ZHAO Y L,YANG S C,CHEN Z J,et al. Contents of total anthocyanins and total saponins as well as composition of saponin monomers of purple and green Notoginseng Radix et Rhizome [J]. Chin Med Mat(中药材),2014,37(10):1749-1753.
[11] YANG Q,WANG W Q,WEI S L,et al. Comparison contents between liquiritin and glycyrrhizic acid in different variant Glycyrrhiza uraleusis [J]. Chin Tradit Herb Drugs (中草药),2007,38(7):1087-1090.
[12] WU J R. Studies on endangerous wild Orchids and its relationship with mycorrhizal fungi in Yunnan province[D]. Nanjing:Nanjing Forestry University,2005.
[13] CHEN X M,YAN H L,WANG C L,et al. Effects of mycorrhizal fungus Mycena sp. on the growth and polysaccharide properties of Dendrobium officinale[J]. Sci China Life Sci (中国科学:生命科学),2016,46(7):872-879.
[14] Ch.P(2015)Vol Ⅳ(中国药典2015年版. 四部)[S]. 2015:202.
[15] CHEN X M,WANG F F,WANG Y Q,et al. Discrimination of the rare medicinal plant Dendrobium officinale based on naringenin, bibeaizyl, and polysaccharides[J]. Sci China Life Sci (中国科学:生命科学),2012,42(12):1002-1009.
[16] LI Y,WANG C L,WANG F F,et al. Phenolic components and flavanones from Dendrobium candidum[J]. Chin Pharm J (中国药学杂志),2010,45(13):975-979.
[17] LI Y,WANG C L,GUO S X,et al. Two new compounds from Dedrobiuru caudidum[J]. Chem Pharm Bull,2008,56:1477-1479.
[18] LI Y,WANG C L,WANG F F,et al. Chemical constituents of Dendrobium candidum[J]. China J Chin Mater Med (中国中药杂志),2010,35(13):1715-1719.
[19] ZHANG L,ZHEN X L,QIU D S,et al. Correlation analysis of major agronomic characters and the polysaccharide contents in Dendrobium officinale[J]. Chin Med Mat (中药材),2013,36(10):1573-1576.
[20] MAO L Z. Photosynthetica characters and medicinal ingredients of Dendrobium candidum under different cultivation conditions [D]. Hangzhou:Zhejiang Normal University,2008.
[21] BAO X Q,WU Z G,YANG P C,et al. Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis[J]. Shanghai J Tradit Chin Med (上海中医药杂志),2015,49(2):86-89.
[22] YAN H L,CHEN X M,LIAO F Y,et al. Effects of mycorrhizal fungi on the growth and polysaccharide accumulation of Dendrobium nobile Lindl seedlings [J]. Chin Pharm J (中国药学杂志),2016,51(17):1450-1454.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家自然科学基金资助项目(81573594);中医药行业科研专项资助项目(201407005)
{{custom_fund}}
PDF(1029 KB)
